Methodologyīefore setting out to do stereology, the researcher needs to acquire data such as the length, surface area, and volume (which is usually done within the stereology program you’ll be using).Īdditionally, it is necessary to set certain criteria for the sample so that the most specific and accurate data can be acquired. Stereology provides quantitative data and tends to provide unbiased results, but as with all types of scientific research, biases and inaccuracies do occur, the primary reason for repeating any study. It involves many variables such as the ‘dissector principle’, ‘physical dissector, variation analyses and ‘guard volume’. Stereology can be used to estimate cell population, determine cell size, regional volume, area fraction and surface area. If that all sounds a bit too much like materials science, then for us microscopists, it’s really about the review of three-dimensional objects (mainly tissues) by making horizontal and vertical incisions. According to the International Society for Stereology, the area of scientific study encompassed by this term is that which analyzes solids.
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